Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 23;7:9192. doi: 10.1038/s41598-017-09078-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The Heatmap of CBL mutations. (A) PamGene analysis was performed to detect the RTK phosphorylation difference between CBL WT and mutants. A549 CBL isogenic cells were treated with MET inhibitor SU11274. After treatment with SU11274, and in comparison with CBL WT, significantly changed peptides were shown as a heatmap. Red color represents the signal upregulation and the blue color represents the signal downregulation. (B) The protein expression of common targets in three CBL mutations was validated by immunoblotting. Protein expression was quantified and indicated with the fold change numbers shown below each immunoblot in comparison with WT. Each protein lysates of separated blot of were collected in the same time period for and the lysates were loaded in one gel per antibody staining. (C) Significantly different peptides were used to analyze affected signaling pathways by Ingenuity Pathway Analysis (IPA). PI3K/AKT, ephrin A, ERK/MAPK, and other signaling pathway were involved especially MET/HGF signaling pathway.