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. 2017 Aug 23;7:9261. doi: 10.1038/s41598-017-08965-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OVX led to greater concentrations of liver triglycerides (a). Intraperitoneal glucose tolerance test was performed at 19wk post-surgery and glucose (b) and insulin (c) changes over time was plotted. Corresponding glucose area under the curve (AUC) (d) and Matsuda Index (e) was calculated from ovariectomized (OVX) vs. sham-operated (SHM) rats bred for low-running capacity fed either a soy-rich or soy-free diet. Data were analyzed using the Mixed Models procedure of SAS 9.3 with diet and surgery being fixed effects and animal ID being a random effect. When a main effect was significant, post hoc pairwise comparisons were performed using Tukey’s multiple comparison tests. Data are least-squares means ± SEM, n = 4–10. ⁺Denotes main surgery effect (OVX vs. SHM); *Denotes main diet effect (Control vs. Soy), P < 0.05.