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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Nat Biotechnol. 2017 May 1;35(6):551–560. doi: 10.1038/nbt.3854

Figure 4. Cellular populations during motorneuron differentiation.

Figure 4

a scTDA identifies four transient populations in mESC differentiation into MNs. Represented is the topological representation (colored by mRNA levels) of four groups of low-dispersion genes; pluripotent, precursor, progenitor and post-mitotic populations. In total, 488 genes were assigned to one of these four populations based on their expression profiles in the topological representation.

b. Reconstructed expression timeline for each of the four groups of low-dispersion genes.

c. Validation by detection of state-specific cell surface markers identified by scTDA. Topological representation (colored by mRNA levels) of surface proteins Pecam1, Ednrb and Slc10a4, and immunostaining of cultured EBs is shown. The scale bar in the immunostaining images denotes a length of 50 μm. Details of three regions are presented on the right. For reference, the topological representation colored by mRNA levels of the Mnx1≳eGFP reporter is also shown.

d. In vivo validation of the motor neuron surface marker Slc10a4. Spinal cord section of an E9.5 mouse immunostained for Slc10a4 (red). The pool of motor neurons is also marked by Mnx1≳eGFP expression (green). The scale bar denotes a length of 50 μm.