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. 2017 Jun 30;292(33):13645–13657. doi: 10.1074/jbc.M117.794099

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 8. — EPR analysis of the resting CYP121 enzyme (250 μm) reacting with peracetic acid (2 mm). The resting CYP121 enzyme is present as a heterogeneous low-spin ferric heme species (black trace). CYP121 was mixed in a 1:1 ratio and quenched in liquid ethane after reaction times of 100 ms (navy trace), 2 s (blue), and 20 s (cyan). The reaction generated at least one high-spin species at g = 6.67, 5.77, and 2 that increased in intensity after longer reaction times. EPR spectra were obtained at 5 K, 9.6-GHz microwave frequency, and 1-milliwatt microwave power. mT, milliteslas.