Figure 3.

The effect of papain‐immunization on WT and R‐Ras‐deficient mice. Wild type (WT) or R‐Ras deficient (R‐Ras KO) mice were treated with papain (500 μg/animal, subcutaneous injection on day 0 and day 14). Animals were euthanized either on day 21 or day 16. IgE concentration of blood serum was measured using ELISA on (A) day 21 or (B) day 16. Experiment in A was done twice with four mice of each genotype and in B the experiment was done four times with 4–5 mice of each genotype. (C and D) LNs (n = 4) and spleens (n = 5) were collected from papain‐immunized (day 16) wild type (WT) or R‐Ras deficient (R‐Ras KO) mice and dendritic cells were enriched as in Figure 1. (C) The amount of all LN MHCII + CD11c + dendritic cells (DC all) and their distribution between conventional (convDC) and migratory (migrDc). (D) The amount of MHCII + CD11c + dendritic cells in the spleen. (E) Expression of IL‐4Rα in CD4 and CD8 T cells of WT and R‐Ras KO mice (n = 5 each genotype) on day 16 after papain immunization. (F) Percent of IL‐33R positive cells of CD4 T cells from WT and R‐Ras KO mice on day 16 after papain immunization. Mean values with SEM are marked in the scatter dot plots. Each dot represents an individual animal. *p < 0.05 (unpaired t‐tests).