Figure 5.

Analysis of RT-PCR products by capillary electro-phoresis following transient transfection of EGFP plasmids into K562 cells. A) pEGFP C1. B) pEGFP C1 + I^wt, C) pEGFP C1+ I^1-110. Peaks “a” and “f” correspond to lower (15 bp) and upper (1500 bp) internal markers in capillary electrophoresis. Peak “b” corresponds to β-actin. Peak “c” corresponds the correctly spliced product (140 bp). Peak “d” in panel C corresponds to the aberrantly spliced product (159 bp). Percentages (19.6% and 80.4%) in panel C correspond to correctly and aberrantly product, respectively. Peak “e” in panel B and C indicate to pre mRNA in pEGFP C1 + I^wt and pEGFP C1+ I^1-110 (270 bp)