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. 2017 Aug 23;16:350. doi: 10.1186/s12936-017-1996-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ROC curve for dried blood spot (DBS) Plasmodium falciparum histidine-rich protein 2 (HRP2) ELISA when compared to clinical microscopy values for patient samples from rural Zambia. Sensitivity of the assay was 94% (CI 89–97%) and specificity was 89% (CI 79–95%) when the threshold was set at 3.5 pM HRP2