Fig. 4.

Enhancer-like intergenic peaks produce short RNAs which positively correlates with the expression of nearest gene. a Distribution of RNA-sequencing reads is plotted over the intergenic peaks and 500 strongly and weakly expressed promoters. Average distribution of RNA-sequencing reads suggests that intergenic peaks produce short (150–300 bps) bi-directional transcripts. b Most of the enhancer-like element RNAs do not exhibit protein coding potential. c Only 7 of the enhancer-like element RNAs overlap with known non-coding RNAs suggesting that enhancer-like element RNAs are novel non-coding RNAs. d The consensus motif at the enhancer-like element is different than the motif found at the highly expressed promoters indicating involvement of different set of transcription factors. e Correlation in the expression of enhancer-like element RNAs from the intergenic peaks is calculated with the expression of nearest gene in a stage specific manner. Enhancer-like element RNAs produced from intergenic regulatory peaks showed moderately positive correlation with the expression of nearest neighbourhood gene