Fig. 10.

a Alignment of amino acid sequences of different XIs. RsXI-C1, novel XI cloned from the cDNA library of symbiotic protists of R. speratus; PiXI, XI of Piromyces sp. E2; CpXI, XI of C. phytofermentans; LlXI, XI of L. lactis. Amino acid sequence alignment was carried out using the genetic information analysis software GENETIX version 10. The shaded column indicates the point mutation site. b Xylose fermentation by recombinant yeast strains expressing mutated PiXI, CpXI, and LlXI. Xylose consumed after 72-h fermentation under microaerobic condition was shown. Xylose fermentation was carried out as described in "Methods". In all cases, initial cell density (absorbance at 600 nm, OD₆₀₀) was 10.0. Error bars represent standard deviations of biological tetraplicates. Statistical significance was determined using Student’s t test. *P < 0.05, significant difference. n.s. no significant difference