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. 2017 Aug 23;10:203. doi: 10.1186/s13068-017-0890-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Xylose fermentation by recombinant yeast strains expressing single amino acid substitution mutants of RsXI-C1. Xylose consumed by S. cerevisiae cells expressing the indicated RsXI-C1 point mutants, obtained by site-saturation mutagenesis of Asn-337, after 72-h fermentation under microaerobic condition. Xylose fermentation was carried out as described in "Methods". In all cases, initial cell density (absorbance at 600 nm, OD₆₀₀) was 1.0. Error bars indicate standard deviations of biological duplicates or triplicates