Table 1. Intra-assay (test precision) and inter-assay variation (test variability) of LightCycler real-time RT–PCR.
| Intra-assay variation (n = 3) | Inter-assay variation (n = 3) | |||
| |
Mean CP |
CV |
Mean CP |
CV |
| TyrA (sample) |
15.020 |
0.06% |
15.311 |
3.58% |
| TyrA (control) |
20.303 |
0.09% |
20.426 |
2.98% |
| PyrB (sample) |
16.031 |
2.16% |
16.289 |
3.91% |
| PyrB (control) |
11.720 |
1.32% |
12.011 |
3.18% |
| Gst (sample) |
14.533 |
0.65% |
14.371 |
2.26% |
| Gst (control) | 14.277 | 0.11% | 13.997 | 2.62% |
Determination of variation was done in 20 ng reverse transcribed total RNA. Test variation is based on CP variation and expressed as mean CP with CV.