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. 2017 Aug 23;17:144. doi: 10.1186/s12870-017-1092-5

Fig. 6.

Fig. 6

Yeast-1-hybrid analysis of RAP2.4 transcription factor binding to the 2CPA and tAPx-I promoter. The growth and vitality of transformed yeast Y187 cells was tested on YPD-plates lacking leucine and tryptophan (−L/−T). For the interaction tests, the yeast cell suspensions were tested on leucine, threonine and histidine free YPD plates (−L/−T/−H) for 4 to 6 days. The stringency was increased with 3-AT