Table 1.

The list of primers used in this study and their predicted targets

Original primer name	Name given	Ref.	Targets in 3D7 [16]	Predicted NF54 target genes [16]
dbla_not_var3	gpA1	[16]	All group A var (DBLα1.2, DBLα1.3, DBLα1.4, DBLα1.5, DBLα1.6, DBLα1.7)	PFD0020c, PFA0015c, MAL6P1.314, PFI1820w, PFD1235w, PFE1640w, PF11_0008, PF08_0141, PF11_0521, PF13_0003
A2	gpA3	[38]	Exon2 of group A var
A3	gpA4	[38]	Exon2 of group A var
cidra1.4	dc13	[16]	DC13 group A var (CIDRα1.4)	PF11_0521
cidra1.1	dc8-1	[16]	One group A, DC8-like var (CIDRα1.1)	PFD0020c
dbla_cidra	dc8-2	[16]	Two group B, DC8-like var (DBLα2_CIDRα1)	MAL6P1.316, PF08_0140
dblb12 & dblb3/5	dc8-3	[16]	DC8-like var in group A and B (DBLβ12, DBLβ3)	MAL6P1.316, PF08_0140, PFD0020c, PF13_0003, PF11_0521 PFD1235w
dblg4/6	dc8-4	[16]	DC8-like var in group A and B (DBLγ4, DBLγ6)	PFD0020c, MAL6P1.316, PF08_0140
B1	b1	[38]	Conserved upstream of group B var
C1	c1	[38]	Conserved upstream of group C var
Seryl-tRNA_synthatase		[39]
Fructose_biphosphase aldolase		[39]

“Primer name” is the name of the primer in the original study (see reference column), “Name given” is the name given to the primer in this study. We included the primers gpA3 and gpA4 designed based on 3D7 genome (a clone of NF54) to independently capture group A expression. Primers were previously shown to amplify the 3D7 genes shown in the right-hand column [16]. Gene names in bold are group A var genes. New 3D7 gene names: PFD0020c = PF3D7_0400400; PFA0015c = PF3D7_0100300; MAL6P1.314 = PF3D7_0600400; PFI1820w = PF3D7_0937600; PFD1235w = PF3D7_0425800; PFE1640w = PF3D7_0533100; PF11_0008 = PF3D7_1100200; PF08_0141 = PF3D7_0800200; PF11_0521 = PF3D7_1150400; PF13_0003 = PF3D7_1300300; MAL6P1.316 = PF3D7_0600200; PF08_0140 = PF3D7_0800300