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HER2 copy number assessment in a patient with robust HER2 amplification: A representative image of fluorescence in situ hybridization for patient 2 is shown. Within each nucleus, red color denotes HER2 signal and green color denotes the centromeric probe (D17Z1) for chromosome 17 used as the control. HER2/Cep17 ratio 9.6, average HER2/nucleus 28.3.
