Fig. 2.

Endothelial function. Vascular relaxation to acetylcholine was reduced in both 12- (a) and 24-week-old (b) male offspring of HFD dams compared to controls, but not in either 12- (c) or 24-week-old (d) female offspring. The ratio of phosphorylated to total eNOS expression was reduced in mesenteric arteries from 12- and 24-week-old male offspring of HFD dams compared to controls (e), but not in female offspring (f). Data are expressed as the mean ± SEM, n = 5–10; * p < 0.05, **** p < 0.0001, 1/2-way ANOVA. CO, controls; wo, weeks old. Black box = CO; Grey box = HFDO.