Fig. 3.

Loss of the anticontractile effect of PVAT in 12-week-old offspring of HFD dams. ePVAT exerted an anticontractile effect on the corresponding PVAT-denuded mesenteric artery segments from 12-week-old male (a) and female (e) offspring of control but not HFD dams (males, b; females, f). Pre-incubation of PVAT-denuded male (c) and female (g) control vessels with 10 mmol/L of glucosamine (gluc) decreased the magnitude of contractions to U46619 to levels similar to those in male (d) and female (h) HFD vessels. Data are expressed as the mean ± SEM, n = 10; ** p < 0.01, *** p < 0.001, 2-way ANOVA.