Fig. 6.

Glucosamine (gluc)-enhanced O-GlcNAcylation in PVAT. Pre-incubation with gluc increased total protein O-GlcNAcylation in both control and HFD PVAT from 12- (a) and 24-week-old (b) male and female offspring. Basal AMPK activity was reduced in control PVAT incubated with gluc from 24-week-old male (c) and female (d) offspring. AMPK (e) and phosphorylated AMPK (f) expressions were markedly reduced in male and female PVAT from 12- and 24-week-old HFDO. Representative immunoblots are shown in the upper part of each section; data are expressed as the mean ± SEM, n = 4; * p < 0.05, 1-way ANOVA/paired t test.