Figure 3.

Interaction of FMRP with mRNPs in RRL may modulate the potency of FMRP as a translation inhibitor. (A) Co-immunoprecipitation of FMRP with FXR2P from RRL. Translation reactions were carried out with 250 nM FMRP when indicated (+). Immunoprecipitations with and without anti-M2 beads were carried out as described in Materials and Methods. An aliquot of flow-through, last wash and eluate for each reaction was loaded on a SDS–PAGE gel followed by immunoblot analysis, using anti-FXR2 antibody. (B) Translation inhibition of brain poly(A) RNA with and without pre-exposure to FMRP. Translation yield of each reaction at 10 and 20 min was plotted. The amount of and treatment by FMRP as well as the number of experiments for each treatment are indicated at the bottom. ***, P < 0.001 for translation yield compared to mock-treated reaction; +++, P < 0.001 for translation yield comparing FMRP treatment with and without pre-incubation with mRNA.