Skip to main content
. 2017 May 26;45(13):7760–7773. doi: 10.1093/nar/gkx443

Figure 2.

Figure 2.

Mitochondrial-targeted MmKu decreases mtDNA content in yeast cells. Total DNA was isolated from KT1112 harboring pGalSu9MmKu grown in Raff or Gal for 8 or 32 h and end-point PCR (A) or real-time qPCR (B) was performed to amplify segments of mtDNA and nDNA. An image (A) representative of at least three independent end-point PCR experiments is shown. For real-time qPCR (B), data are represented graphically as the mean ± SD from three independent experiments. * represents P < 0.05. (C) Part of the cultures used for DNA isolation were used to quantitate the percentage of ρ+ colonies, and the averages are shown graphically. Error bars represent SD and * represents P < 0.05. (D) MmKu, Cox4 and Pgk1 expression in KT1112 carrying pGalSu9MmKu grown in Raff or Gal for 8 h and 32 h. An immunoblot representative of three independent experiments is shown. Cox4, cytochrome C oxidase subunit 4, is a mitochondrial protein.