Figure 4.

aPKCζ is required for EGF-dependent MRLC phosphorylation. (A) aPKCζ^−/− and control cells were stimulated with EGF, and at the indicated time point the cells were lysed in SDS-PAGE sample buffer divided into 2 samples that were subjected to Western blot analysis. One sample was probed with anti-MRLC antibodies and the other anti-pMRLC antibodies. Shown are representative Western blots. (B) Densitometry analysis of Western blots. Bands were analyzed by densitometry using ImageJ and the relative amounts of pMRLC to total MRLC are shown. Values represent the mean ± SEM for 5 independent experiments.