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aPKCζ affects the spatial organization of pMRLC. aPKCζ−/− and control cells were subjected to wound scratch assay (A) or seeded on coverslips and stimulated with EGF for 4 min (B). Cells were stained for F-actin, using Rhodamine-Phalloidin, and with anti-pMRLC antibody and secondary antibody conjugated to Alexa flour 488. Bars are 20 μm.
