Figure 4.

TGF-β increases the expression and nuclear localization of ERRα in osteosarcoma cells. (A) MG-63 and HOS cells were treated with or without TGF-β (20 ng/ml) for 24 h, and then the express of protein (A) and mRNA (B) of ERRα, ERα, ERβ, or ERRγ were measured by use of Western blot analysis and qRT-PCR, respectively; (C) MG-63 cells were treated with or without TGF-β (20 ng/ml) for 24 h, the cellular location of ERRα (green) were examined by immunofluorescence staining and nuclei were stained with DAPI (blue). The quantification results were shown in the right column. Data were presented as means ± SD of 3 independent experiments. **p < 0.01 compared with control. Scale bar = 50 μm.