Table 1. Experimentally derived haplotypes for HLA-A.
Reaction sample | Reads† [% trc] | Identified alleles | Barcode clusters | % trc | Found in sample ID | IPD-IMGT/HLA CORRESPONDENCE | External references |
---|---|---|---|---|---|---|---|
8-Plex ID 01-08 | 1.74 M [54.2%] | A | 667 | 14.37% | 01, 03, 04, 05, 08 | A*02:01:01:01 | A*02:01 |
B | 362 | 13.38% | 02, 03, 08 | A*11:01:01:01 | A*11:01 | ||
C | 409 | 10.23% | 02, 05, 07 | A*01:01:01:01 | A*01:01 | ||
D | 215 | 5.64% | 04, 06 | A*29:02:01:01, A*29:02:01:02 | A*29:02 | ||
E | 134 | 3.98% | 01 | A*03:01:01:01 | No Data | ||
F | 142 | 3.68% | 07 | A*24:02:01:01 | A*24:02 | ||
G | 122 | 2.74% | 06 | A*26:01:01:01, A*26:01:01:03N | A*26:01 | ||
ID 01 | 862 K [53.1%] | E | 744 | 34.81% | 01 | A*03:01:01:01 | No Data |
A | 486 | 18.27% | A*02:01:01:01 | ||||
ID 02 | 865 K [60.9%] | C | 563 | 33.17% | 02 | A*01:01:01:01 | A*01:01 |
B | 419 | 27.44% | A*11:01:01:01 | A*11:01 | |||
ID 03 | 1.05 M [72.9%] | B | 783 | 42.37% | 03 | A*11:01:01:01 | No Data |
A | 730 | 30.93% | A*02:01:01:01 | ||||
ID 04 | 833 K [57.3%] | D | 369 | 32.65% | 04 | A*29:02:01:01, A*29:02:01:02 | A*29:02 |
A | 423 | 27.11% | A*02:01:01:01 | A*02:01 | |||
ID 05 | 1.06 M [63.5%] | C | 552 | 37.74% | 05 | A*01:01:01:01 | A*01:01 |
A | 524 | 32.13% | A*02:01:01:01 | A*02:01 | |||
ID 06 | 1.05 M [64.1%] | D | 460 | 30.9% | 06 | A*29:02:01:01, A*29:02:01:02 | A*29:02 |
G | 433 | 27.57% | A*26:01:01:01, A*26:01:01:03N | A*26:01 | |||
ID 07 | 857 K [63.6%] | F | 718 | 33.13% | 07 | A*24:02:01:01 | A*24:02 |
C | 787 | 30.50% | A*01:01:01:01 | A*01:01 | |||
ID 08 | 1.02 M [69.0%] | B | 880 | 44.36% | 08 | A*11:01:01:01 | A*11:01 |
A | 667 | 24.58% | A*02:01:01:01 | A*02:01 |
Results for the eight-plex and all singleplex reactions, featuring correspondence to the IPD-IMGT/HLA database and two independent sources (24,25) of haplotyping data. † Reads counts of barcode clusters used for classification of alleles. % trc corresponds to percentage of total read counts. Only matching database entries that have been confirmed and published are included. The table details the data analysis output of all alleles with support from at least two independent barcode clusters.