Skip to main content
. 2001 Jun 1;29(11):2349–2360. doi: 10.1093/nar/29.11.2349

Figure 4.

Figure 4

Distribution of APE2 protein in the nuclei of human cells. (A) APE2 forms nuclear foci in cultured human cells. APE2 protein in HeLa MRV (a and b) and HeLa MR:APE2-HA cells (c and d) were examined by laser scanning fluorescence microscopy, using anti-APE2 pre-adsorbed to TrxA–Sepharose (a and c) or TrxA–APE2–Sepharose (b and d), in combination with Alexa Fluor 488-labeled second antibody. Nuclei were counterstained with TOTO-3 (a–d). Alexa Fluor 488 and TOTO-3 signals are shown in green and blue, respectively. (B) Co-localization of APE2 and PCNA in nuclear foci. HeLa MRV (a–c) and HeLa MR:APE2-HA (d–f) cells were immunostained with anti-APE2/Alexa Fluor 488-labeled second antibody and anti-PCNA/Alexa Fluor 594-labeled second antibody and were subjected to laser scanning fluorescence microscopy. APE2 and PCNA signals are shown in green (a and d) and red (b and e), respectively. In merged images in (c) and (f) co-localized signals are shown in yellow.