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. 2017 May 26;45(13):8014–8025. doi: 10.1093/nar/gkx470

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Yield of (M−nH)ⁿ⁻ ions from ESI of 1:1:1 mixtures of 15 nt RNAs 1, 2 and 3 in 1:1 H₂O/CH₃OH versus total RNA concentration with 20 mM piperidine (circles), 20 mM piperidine and 20 mM imidazole (diamonds), or 20 mM ammonium acetate (triangles) as additive. Open symbols represent data from individual spectra and filled symbols show average values with standard deviations as error bars. Color coding refers to the number of m⁵U residues: gray for 0 (RNA 1), violet for 2 (RNA 2) and green for 4 (RNA 3).