Table 1. K D for binding of hnRNP L and RRM domains to VEGF-A CARE and miR-574-3p.
Protein (or domain) | VEGF-A CARE (wild-type) KD (M) | miR-574-3p KD (M) | VEGF-A GAIT element KD (M) |
---|---|---|---|
hnRNP L | 8.5 ± 1.5 × 10−9 | 11.7 ± 3.6 × 10−9 | N.D. |
RRM 1,2 | 7.6 ± 2.1 × 10−9 | 21.4 ± 9.3 × 10−9 | N.D. |
RRM 1,2-H105A | 61.2 ± 18.6 × 10−9 | 91.0 ± 26.6 × 10−9 | N.D. |
RRM 3,4 | 47.9 ± 8.7 × 10−9 | 18.1 ± 8.6 × 10−9 | N.D. |
Biotinylated VEGF-A CARE, miR-574-3p and GAIT element RNA were immobilized on a streptavidin chip for SPR determination of binding of recombinant analyte protein. The KD was calculated using Biaevaluation software. N.D., not detected.