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. 2017 May 17;45(13):7950–7964. doi: 10.1093/nar/gkx440

Table 1. K D for binding of hnRNP L and RRM domains to VEGF-A CARE and miR-574-3p.

Protein (or domain) VEGF-A CARE (wild-type) KD (M) miR-574-3p KD (M) VEGF-A GAIT element KD (M)
hnRNP L 8.5 ± 1.5 × 10−9 11.7 ± 3.6 × 10−9 N.D.
RRM 1,2 7.6 ± 2.1 × 10−9 21.4 ± 9.3 × 10−9 N.D.
RRM 1,2-H105A 61.2 ± 18.6 × 10−9 91.0 ± 26.6 × 10−9 N.D.
RRM 3,4 47.9 ± 8.7 × 10−9 18.1 ± 8.6 × 10−9 N.D.

Biotinylated VEGF-A CARE, miR-574-3p and GAIT element RNA were immobilized on a streptavidin chip for SPR determination of binding of recombinant analyte protein. The KD was calculated using Biaevaluation software. N.D., not detected.