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. 2017 Jun 7;45(13):7581–7592. doi: 10.1093/nar/gkx507

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Stabilization of 5΄ phosphate increases duration of effect and corresponding tissue accumulation of hsiRNA in vivo. (A) Column scatter plots showing Ppib mRNA levels in the livers and kidneys of mice (n = 5 per group) at the indicated times after subcutaneous injection of PBS or 5΄-OH or 5΄-VP hsiRNAs. mRNA levels measured by QuantiGene^® assay were normalized to Hprt mRNA and expressed as a percent of mRNA levels in PBS-treated animals. Significance calculated by ANOVA with Bonferroni΄ s correction, significance was calculated in comparison to PBS-treated animals: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 and ****P ≤ 0.0001. Mean ± SD. (B) Bar graphs showing the concentration of 5΄-OH and 5΄-VP hsiRNA guide strands in liver and kidney, as measured by the PNA-based hybridization assay at the indicated time points after subcutaneous injection. Mean ± SD, N = 50.

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