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A schematic of the protocol used to build models of the chromosome. A 500 base-pair-per-bead (BPB) structure is progressively compacted into the nucleoid volume, defining a path for higher resolution structures to follow. Initial 500 BPB (red) and 5 BPB (blue) ‘blueprint’ structures appear at upper left; the insets for the 5 BPB structure show supercoiled duplexes in part of a single plectoneme. Full details of the process are given in Supplementary Data.
