Table 2:
Composition of control populations and comparison of phylotyping and shotgun metagenomics abundance measurements
| Control | Total | ITS abundance | Shotgun | Control | Total | ITS abundance | Shotgun | ||
|---|---|---|---|---|---|---|---|---|---|
| Strain | Species | mix 1 | OTUs | (ratio)a | abundance (ratio)a | mix 2 | OTUs | (ratio)a | abundance (ratio)a |
| AWRI796 | Saccharomyces cerevisiae | 1 × 106 | 3 | 1 × 106 (1)b | 1 × 106 (1)b | 1 × 108 | 11 | 2 × 108 (1)b | 2 × 108 (1)b |
| AWRI1498 | Saccharomyces cerevisiae | 1 × 104 | 1 × 108 | ||||||
| AWRI1149 | Metschnikowia pulcherrima | 1 × 104 | 7 | 1.9 × 105 (18.6) | 8.8 × 103 (0.9) | 1 × 106 | 7 | 1.1 × 107 (10.5) | 6.5 × 105 (0.7) |
| AWRI1152 | Torulaspora delbrueckii | 1 × 106 | 1 | 7.3 × 105 (0.7) | 4.8 × 105 (0.5) | 1 × 105 | 1 | 6.6 × 104 (0.7) | 4.7 × 104 (0.5) |
| AWRI1157 | Debaryomyces hansenii | 1 × 107 | 1 | 8.4 × 106 (0.8) | 2.9 × 106 (0.3) | 1 × 103 | 1 | 2.4 × 103 (2.4) | 0.0 |
| AWRI1176 | Saccharomyces uvarum | 1 × 103 | 1 | 5.7 × 102 (0.6) | 1.8 × 103 (1.8) | 1 × 105 | 3 | 7.9 × 104 (0.8) | 1.1 × 105 (1.1) |
| AWRI1274 | Haneniaspora uvarum | 1 × 108 | 4 | 1.1 × 108 (1.1) | 1.3 × 108 (1.3) | 1 × 104 | 2 | 6.1 × 104 (6.1) | 4.4 × 104 (4.4) |
aThe ratios are presented as the observed abundance/expected abundance (the total number of cells added to the control mix).
bAll data were internally normalized for comparison by setting the observed abundance of S. cerevisiae to a final ratio of 1.