. 2017 Jun 8;6(7):1–10. doi: 10.1093/gigascience/gix040

Table 4:

ITS amplification primers used in this study

Primer	Sequence (Illumina adaptor^a \| in-line barcode^b \| spacer^c \| ITS primer sequence^d)
BITS-F1-N701	`CCTACACGACGCTCTTCCGATCT\|TAAGGCGA\|\|ACCTGCGGARGGATCA`
BITS-F1-N702	`CCTACACGACGCTCTTCCGATCT\|CGTACTAG\|C\|ACCTGCGGARGGATCA`
BITS-F1-N703	`CCTACACGACGCTCTTCCGATCT\|AGGCAGAA\|TC\|ACCTGCGGARGGATCA`
BITS-F1-N704	`CCTACACGACGCTCTTCCGATCT\|TCCTGAGC\|ATC\|ACCTGCGGARGGATCA`
BITS-R1-N701	`GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT\|TAAGGCGA\|\|GAGATCCRTTGYTRAAAGTT`
BITS-R1-N702	`GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT\|CGTACTAG\|C\|GAGATCCRTTGYTRAAAGTT`
BITS-R1-N703	`GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT\|AGGCAGAA\|TC\|GAGATCCRTTGYTRAAAGTT`
BITS-R1-N704	`GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT\|TCCTGAGC\|ATC\|GAGATCCRTTGYTRAAAGTT`

^aCommon sequence allowing for clustering on the Illumina flow cell.

^b8-bp variable barcode.

^cVariable length spacer (0–3 bp) used to unphase conserved amplicon regions and provide higher-quality sequencing on the Illumina platform.

^dSequences derived from [9].