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. 2017 May 31;45(13):7897–7908. doi: 10.1093/nar/gkx490

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Selective oncogene disruption using Ad/sgEGFR and Ad/Cas9 in vivo. Indels at the wild-type (gray bar) and mutant EGFR alleles (blue bar) in (A) H1975 or (B) A549 tumor xenografts co-injected with Ad/sgEGFR and Ad/Cas9 at day 7, 9, 11 and 31 after the first injection. Tumor-bearing mice were given intratumoral injections of PBS (mock) or 5 × 10¹⁰ Ad VPs (Ad/Cas9 + Ad/empty vector or Ad/sgEGFR + Ad/Cas9) on days 1, 3 and 5. Values represent the mean ± S.E.M. (n= 3 to 4). **P < 0.01. ns: not significant. (C) No off-target indels were detectably induced at eight homologous sites that differed from the on-target sites by up to 3 nt in the human genome. Mismatched nucleotides are shown in blue and PAM sequences in red, On; on-target site, OT; off-target site. Red arrow indicates cleavage position within the 19-bp target sequences. Error bar indicates S.E.M. (n = 3 to 4).