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. 2017 May 10;45(13):7708–7721. doi: 10.1093/nar/gkx373

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Snf2 is downregulated in the meiotically efficient SK1 yeast strain. (A) Western blot for levels of Snf2 protein through a time course of sporulation in the SK1 strain. Pgk1 is an internal control. (B) Quantification of total (exon 2) transcript levels for RPS16A, RPL34B and RPL13A by RT-qPCR relative to scR1 and normalized to t = 0 in WT yeast (SK1 strain) at indicated times after shift to sporulation media. Error bars represent ± 1 SD. (C) Quantification of total transcript levels for Initiator of Meiotic Entry 1 (IME1) by RT-qPCR relative to scR1 and normalized to t = 0 in WT yeast (SK1 strain) at indicated times after shift to sporulation media. Error bars represent ± 1 SD. (D) Quantification of total transcript levels for MER1 by RT-qPCR relative to scR1 and normalized to t = 0 in WT yeast (SK1 strain) at indicated times after shift to sporulation media. Error bars represent ± 1 SD. (E). Expression and splicing of Mer1-regulated meiotic transcript MER2 at indicated times after shift to sporulation media. PCR products representing the spliced and unspliced forms are indicated.