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. 2017 Jun 21;45(13):7602–7614. doi: 10.1093/nar/gkx546

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Potent, affinity-dependent silencing mediated by the targeted siRNA carriers. A431-d2EGFP cells were transfected with p19 carriers loaded with GFP siRNA. The concentration of C225.2/PFO^T490A,L491V was fixed at either 5 or 0.5 nM. Transfection was performed for 6 h, and GFP expression or cell viability was measured at 24 h. The collected data were normalized to that of control cells treated only with the corresponding concentration of C225.2/PFO^T490A,L491V. (A) Transfection using p19-E18 carriers. Shown is the average of three independent measurements. Normalized data was fitted to a four-parameter logistic curve to obtain the EC₅₀ of silencing. (B) Identical experiment to (A) but with p19-E6 carriers. (C) Cell viability was measured using the WST-1 reagent following the transfection procedures of (A) and (B). (D) Identical experiment to (A) but with untargeted p19 carriers. Shown is the average of two independent measurements.