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. 2017 Jun 21;45(13):7602–7614. doi: 10.1093/nar/gkx546

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Higher carrier affinity for siRNA improves both uptake into the cell and the downstream efficiency of delivery within the cell. (A) The time- and concentration-dependent uptake of siRNA into. A431-d2EGFP cells mediated by each p19-E18 clone. Denoted concentrations are those of the p19-E18/siRNA complex. The p19-E18 clones were loaded with fluorescently labeled siRNA (Seq I), and the background-subtracted fluorescence in cells was converted to number of siRNAs using calibration beads. Shown are the averages of two independent measurements. (B) The silencing potencies mediated by p19-E18 and p19^N15K,G16R-E18 per internalized siRNA. A soluble competitor for EGFR (sumo-E18) was used to titrate the number of siRNA complexes that are internalized. Fluorescently labeled siRNA (Seq I) was used to measure siRNA uptake after 6 h, and GFP siRNA was used to measure GFP knockdown in an analogous setting (Supplementary Figure S12). Shown are the averages of three independent measurements.