Figure 1.

Examples for a likely mis-annotated gene in some (provisional) NCBI Reference Sequence (NCBI RefSeq) entries encountered during this work. Exon–exon junction of the chloroplast rpl2 gene as translated from NCBI RefSeq CDS entries (left) and comparison with the corresponding entries in the GeSeq internal reference sequences database (right; see Supplementary Table S1 for GeSeq names and GenBank IDs). Suspected annotation errors are labeled in black. Some NCBI RefSeq records lack annotation of given genes (‘no annotation’, cf. Brassica rapa) or introns resulting in no or truncated CDS (Oryza sativa ssp. indica and Saccharum), respectively. Inconsistent exon borders result in likely erroneous insertions or deletions of one or several codons (Ginkgo biloba, Medicago truncatula, Ricinus communis, Sorghum bicolor and Selaginella moellendorffii).