Table 1.
Pathological composition and characteristics of the analyzed mutational load patient cohort
| Pathology | All Patients | GBM | Gliosarcoma | High Grade NOS | Grade III | Grade II | Grade I | Other |
|---|---|---|---|---|---|---|---|---|
| WHO grade | I + II + III + IV | IV | IV | III/IV | III | II | I | ? |
| Number of patients (%) | 327 | 198 | 10 | 16 | 34 | 45 | 5 | 19 |
| Parameters | ||||||||
| Sex, no. (%) | ||||||||
| Male | 179 (54.7) | 110 (55.6) | 6 (60.0) | 8 (50.0) | 19 (55.9) | 23 (51.1) | 3 (60.0) | 10 (52.6) |
| Female | 148 (45.3) | 88 (44.4) | 4 (40.0) | 8 (50.0) | 15 (44.1) | 22 (48.9) | 2 (40.0) | 9 (47.4) |
| Age, mean y (range) | 52.1 (0-85) | 56.6 (4-85) | 52.9 (35-63) | 53.9 (28-81) | 46.7 (21-73) | 42.4 (18-78) | 19.4 (0-56) | 44.3 (15-74) |
| <50 y | 121 (37.0) | 44 (22.2) | 3 (30.0) | 6 (37.5) | 18 (52.9) | 34 (75.6) | 4 (80.0) | 13 (68.4) |
| ≥50 y | 206 (63.0) | 154 (77.8) | 7 (70.0) | 10 (62.5) | 16 (47.1) | 11 (24.4) | 1 (20.0) | 6 (31.6) |
| IDH1 mutation | ||||||||
| Wild type | 248 | 182 | 10 | 12 | 10 | 16 | 5 | 13 |
| Mutated | 79 | 16 | 0 | 4 | 24 | 29 | 0 | 6 |
| Mutational load | ||||||||
| <10 mutations/1.4 Mb (low) | 279 | 171 | 8 | 13 | 25 | 41 | 5 | 16 |
| 11-20 mutations/1.4 Mb (moderate) | 33 | 20 | 1 | 1 | 5 | 4 | 0 | 2 |
| >20 mutations/1.4 Mb (high) | 15 | 7 | 1 | 2 | 4 | 0 | 0 | 1 |
| Mean TML ± SD | 10.2 (±26.2) | 9.8 (±27.7) | 18.8 (±38.2) | 11.1 (±17.5) | 11.4 (±11.9) | 6.7 (± 2.8) | 4.8 (±2.7) | 17.7 (±51.2) |
| PD-1/PD-L1 expression | ||||||||
| PD-1 on TILs, no. / total no. (%) | ||||||||
| Positive | 59/152 (38.8) | 43/94 (45.7) | 3/4 (75.0) | 3/8 (37.5) | 14/16 (87.5) | 1/16 (6.2) | 1/2 (50.0) | 6/12 (50.0) |
| PD-L1 on tumor cells, no. / total no. (%) | ||||||||
| Positive | 24/295 (8.1) | 19/189 (10.1) | 3/10 (30.0) | 1/16 (6.2) | 2/16 (12.5) | 0/41 (0) | 0/5 (0.0) | 1/18 (5.5) |
| MMR protein expression | ||||||||
| MLH1, no. / total no. | ||||||||
| Expression | 33/35 | 28/30 | 0/1 | 2/2 | 0/1 | 3/3 | 1/1 | 3/3 |
| MSH2, no. / total no. | ||||||||
| Expression | 33/35 | 27/30 | 0/1 | 2/2 | 0/1 | 3/3 | 1/1 | 3/3 |
| MSH6, no. / total no. | ||||||||
| Expression | 31/34 | 26/30 | 0/1 | 2/2 | 0/1 | 2/2 | 1/1 | 3/3 |
| PMS2, no. / total no. | ||||||||
| Expression | 32/35 | 28/30 | 0/1 | 2/2 | 0/1 | 3/3 | 1/1 | 2/3 |