Silver-stained 5% polyacrylamide gels showing: A) ITS1-PCR: Lane M—Promega standard molecular weight ladder (100 bp), Lane 1—positive control—amplicon from DNA of Leishmania infantum promastigotes, Lanes 2–15—amplicons from DNA sandfly samples collected in Caxias, Maranhão, Brazil, Lane 16—negative control; B) ITS1-PCR-RFLP (HaeIII digestion): Lane M—Invitrogen standard molecular weight ladder (25 bp), Lanes I-IX—DNA from positive controls, I—L. amazonensis, II—L. mexicana, III–L. guyanensis, IV- L. braziliensis, V–L. lansoni, VI–L. naiffi, VII—L. shawi, VIII—L. infantum, IX—L. infantum/L. braziliensis, Lanes 3–55—DNA from phlebotomine samples C) ITS1-PCR-RFLP (Hae III digestion): Lane M: Invitrogen standard molecular weight ladder (25 bp), Lanes 56–209—DNA from phlebotomine samples.