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. 2017 Aug 24;12(8):e0183605. doi: 10.1371/journal.pone.0183605

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© 2017 Kudumala et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Comparison of phenotypes when Lis1 was knocked down in the GF during its development (R91H05) or after its development (GF-Split-Gal4) with RNAi (UAS-Lis1^RNAiH) and CRISPR (UAS-Lis1^gRNA). All images show maximum intensity projections of confocal image stacks. GFs of adult flies were labeled by TRITC-dextran injections (magenta) and displayed together with immunohistochemically labeled Nrg¹⁸⁰ (green) in the VNC (upper rows). Co-localization of both labels appears white. The lower rows display immunohistochemically labeled Nrg¹⁸⁰ separately as gray scale images. Scale bars are 20 μm. Localization of Nrg¹⁸⁰ (anti-Nrg-BP104, green) in wild type and lis1 mutant backgrounds. Vesicular accumulations of Nrg¹⁸⁰ in stunted and normal sized terminals are indicated by arrows. A large vacuole is indicated by an arrowhead.