Table 2. Linearity and lower limits of detection and quantification for TH and TAM spiked lysed Hep G2 and PCCL3 cell extracts in 24- and 6-well plates respectively.

	Hep G2			PCCL3
Compound	R2 Range	LLOQ [nM]	LLOD [nM]	R2 Range	LLOQ [nM]	LLOD [nM]
T0	0.995–0.999	*	*	0.996–1.000	0.050	0.025
3-T1	0.996–1.000	0.063	0.031	0.997–1.000	0.050	0.025
3'-T1	0.995–1.000	0.031	0.016	0.997–1.000	0.050	0.025
3,5-T2	0.996–1.000	0.125	0.063	0.996–1.000	0.050	0.025
3,3'-T2	0.996–1.000	0.063	0.031	0.998–0.999	0.100	0.025
3',5'-T2	0.995–0.999	0.031	0.016	0.997–1.000	0.050	0.025
T3	0.995–1.000	0.063	0.016	0.997–0.999	0.040	**
rT3	0.997–1.000	0.063	0.031	0.998–0.999	0.100	0.010
T4	0.996–1.000	0.063	0.016	0.997–0.999	0.040	**
T0AM	0.995–1.000	0.125	0.063	0.996–0.999	0.500	0.025
3-T1AM	0.996–1.000	0.031	0.016	0.997–0.999	0.100	0.020
3,5-T2AM	0.996–1.000	0.063	0.031	0.998–1.000	0.050	0.025
T3AM	0.994–1.000	0.063	0.031	0.997–0.999	0.050	0.010
rT3AM	0.995–0.999	0.063	0.031	0.997–0.999	0.050	0.010
T4AM	0.996–0.998	0.250	0.125	0.995–0.999	0.500	0.100
T0Ac	-	-	-	0.995–0.999	1.000	0.330
3-T1Ac	-	-	-	0.996–0.999	1.000	0.600

R²: Pearson coefficient, 1/x weighted linear regression. LLOQ: lower limit of quantification LLOD: lower limit of detection.

*LLOD/LLOQ not quantifiable (lysed cell extracts contained ca. 0.10 nM T₀)

** LLOD not quantifiable (lysed cell extracts contained ca. 0.010nM T₄ and T₃)