Table 6. Intra-day (n = 8) accuracy and precision for TH, TAM and TAc in spiked lysed PCCL3 cell extracts in 6-well plates.

	0.10			12.5			40.0
Compound	Mean Calculated Concentration	% Relative Standard Deviation	% Accuracy	Mean Calculated Concentration	% Relative Standard Deviation	% Accuracy	Mean Calculated Concentration	% Relative Standard Deviation	% Accuracy
T0	0.10	8.6	103	12.7	3.8	102	39.8	1.4	99
3T1	0.10	12.6	99	12.5	3.2	100	39.3	2.2	98
3'T1	0.09	12.7	94	12.8	2.8	103	40.1	1.1	100
3,5T2	0.10	5.3	100	12.9	2.4	103	39.3	2.2	98
3,3'T2	0.10	9.1	98	12.8	1.2	102	40.5	1.3	101
3',5'-T2	0.11	6.6	108	12.6	2.4	101	39.9	1.9	100
T3	0.11	8.6	106	12.7	1.9	102	40.4	1.8	101
rT3	0.11	7.0	106	12.2	1.5	97	38.7	3.6	97
T4	0.10	4.5	103	12.6	1.6	101	39.9	1.6	100
T0AM	0.09	14.0	89	12.5	1.2	100	39.0	1.7	98
3T1AM	0.10	5.6	95	12.4	0.8	99	39.9	1.0	100
3,5T2AM	0.09	7.9	94	12.1	3.2	97	39.5	1.5	99
T3AM	0.10	8.8	104	11.1	17.0	89	39.4	3.2	98
rT3AM	0.10	6.3	101	11.1	18.8	89	38.4	4.2	96
T4AM	<LLOQ			11.4	19.4	91	36.9	6.1	92
T0Ac	<LLOQ			12.6	2.5	101	39.3	3.5	98
T1Ac	<LLOQ			13.6	4.4	109	40.2	3.7	101