Table 7. Inter-day (n = 8) process efficiency, matrix effect and recovery for TH and TAM spiked lysed Hep G2 cell extracts in 24-well plates.

Compound	0.10 nM									12.5 nM									40.0 nM
	PE	±	%RSD	ME	±	%RSD	RE	±	%RSD	PE	±	%RSD	ME	±	%RSD	RE	±	%RSD	PE	±	%RSD	ME	±	%RSD	RE	±	%RSD
2H4-3-T1AM	69	±	12	84	±	11	82	±	10	69	±	10	82	±	9	81	±	13	71	±	10	86	±	6	83	±	13
13C6-3,3'T2	73	±	8	87	±	8	85	±	8	74	±	8	83	±	5	85	±	11	75	±	9	87	±	6	86	±	11
13C6-T3	78	±	8	91	±	7	86	±	8	80	±	8	88	±	5	89	±	11	78	±	10	88	±	8	88	±	12
13C6-rT3	67	±	10	84	±	11	80	±	7	69	±	8	79	±	8	85	±	10	70	±	10	84	±	8	84	±	12
13C6-T4	67	±	13	84	±	13	80	±	10	69	±	11	80	±	10	84	±	11	68	±	10	81	±	10	85	±	12
T0	148	±	17	170	±	15	87	±	9	71	±	8	91	±	7	76	±	12	72	±	11	92	±	6	78	±	13
3-T1	78	±	10	85	±	8	91	±	8	74	±	10	88	±	8	83	±	13	75	±	10	90	±	7	84	±	11
3'-T1	74	±	9	84	±	12	88	±	10	70	±	9	84	±	7	81	±	10	73	±	10	87	±	7	84	±	13
3,5-T2	<LLOQ			<LLOQ			<LLOQ			81	±	10	96	±	8	83	±	12	80	±	11	94	±	6	86	±	10
3,3'-T2	75	±	9	82	±	10	92	±	10	73	±	9	87	±	6	84	±	10	76	±	13	88	±	6	86	±	11
3',5-'T2	70	±	13	80	±	13	88	±	11	67	±	9	83	±	9	81	±	11	71	±	10	86	±	7	82	±	11
T3	81	±	13	89	±	10	91	±	10	72	±	11	87	±	8	81	±	12	75	±	11	90	±	7	84	±	11
rT3	75	±	10	86	±	11	88	±	10	68	±	8	85	±	7	83	±	10	71	±	10	87	±	6	82	±	11
T4	72	±	20	83	±	18	86	±	12	64	±	15	83	±	13	79	±	10	68	±	15	86	±	9	79	±	12
T0AM	<LLOQ			<LLOQ			<LLOQ			74	±	13	87	±	9	83	±	13	77	±	13	90	±	7	84	±	12
3-T1AM	75	±	13	87	±	15	87	±	14	69	±	11	87	±	8	78	±	13	72	±	12	90	±	5	80	±	13
3,5-T2AM	71	±	13	84	±	12	85	±	14	64	±	14	85	±	11	74	±	12	67	±	14	86	±	8	78	±	15
T3AM	64	±	26	77	±	33	85	±	19	61	±	15	79	±	16	80	±	11	66	±	13	84	±	9	78	±	16
rT3AM	63	±	14	80	±	14	79	±	19	59	±	15	79	±	11	79	±	11	62	±	16	81	±	8	76	±	18
T4AM	<LLOQ			<LLOQ			<LLOQ			51	±	21	70	±	11	79	±	10	60	±	21	74	±	15	73	±	24

Values were obtained by spiking samples with in-house prepared TH standard solutions. Lysed cell extracts contained ca. 0.10 nM T₀, thus results at 0.10 nM are skewed.