Table 9. Mass balance in cells/supernatants for THM after incubation of mono-iodinated THM with PCCL3 rat thyrocytes.
| Incubation time | 5min | 10min | 30min | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Incubation compound (pmol) | Compound | Cells | Supernatants | Total | Cells | Supernatants | Total | Cells | Supernatants | Total | ||||||||||||
| pmol | (%) | | | pmol | (%) | | | pmol % | pmol | (%) | | | pmol | (%) | | | pmol % | pmol | (%) | | | pmol | (%) | | | pmol % | ||
| T1 (775.9) | T1 | 32 | (4%) | | | 498 | (62%) | | | 66% | 34 | (4%) | | | 502 | (63%) | | | 67% | 39 | (5%) | | | 507 | (63%) | | | 69% |
| T0 | 1 | (0.1%) | | | 0.3 | (0.03%) | | | 1 | (0.2%) | | | 1 | (0.1%) | | | 2 | (0.2%) | | | 3 | (0.4%) | | | ||||
| 3-T1AM (812.7) | 3-T1AM | 238 | (29%) | | | 323 | (40%) | | | 69% | 276 | (34%) | | | 333 | (41%) | | | 76% | 334 | (41%) | | | 329 | (40%) | | | 84% |
| T0AM | 1 | (0.2%) | | | 1 | (0.1%) | | | 3 | (0.4%) | | | 2 | (0.2%) | | | 3 | (0.3%) | | | 6 | (0.8%) | | | ||||
| 3-T1Ac | 1 | (0.1%) | | | nd | (0%) | | | 1 | (0.2%) | | | nd | (0%) | | | 1 | (0.1%) | | | 9 | (1.2%) | | | ||||
| 3-T1Ac (777.6) | 3-T1Ac | 4 | (0.6%) | | | 596 | (77%) | | | 77% | 4 | (0.5%) | | | 599 | (77%) | | | 79% | 3 | (0.3%) | | | 632 | (81%) | | | 84% |
| T0Ac | 1 | (0.1%) | | | nd | (0%) | | | 1 | (0.2%) | | | 8 | (1.1%) | | | 1 | (0.1%) | | | 14 | (1.9%) | | | ||||
pmol: absolute amount in cell lysate (0.4 mL) or supernatant (1.5 mL), (%): relative % pmol for individual compound with respect to pmol of incubation compound, Total pmol %: summation of % pmol for all compounds detected in cell lysates or supernatants with respect to pmol of incubation compound. % pmol values were not adjusted to account for relative recoveries.