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. 2017 Aug 19;19(3):415–424. doi: 10.22074/cellj.2017.4364

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Fig.3 — Measurement of oxidative burst and nitric oxide production in macrophages. A. Evaluation of macrophage (Mφ) respiratory burst after activation by tetradecanoylphorbol acetate (TPA) and B. Nitric oxide (NO) production of macrophages after challenge with lipopolysaccharide (LPS). Mesenchymal stem cells (MSCs) were pulsed with different concentrations of caffeine [0.1 (T0.1), 0.5 (T0.5), and 1 (T1) mM] for 72 hours. Then, MSCs were cultured for 24 hours without treatment after which we collected the conditioned medium of MSCs. Macrophages were incubated for 24 hours with the conditioned medium of MSCs pulsed with caffeine or the conditioned medium of MSCs alone (T0). The respiratory burst and NO production of macrophages were assessed by thenitroblue tetrazolium (NBT) reduction assay and Griess method, respectively.

*; P<0.05, **; P<0.01, ***; P<0.001 vs. Mφ, #; P<0.01, and ##; P<0.001 vs. T0.