Table 1. Strains and plasmids used at this study.

	Characteristics	Reference or Supplier
Strains
P. aeruginosa PAO1	Wild-Type Amp R	[16]
P. aeruginosa-estA	P. aeruginosa PAO1 containing plasmid pUCP26estA	This study
E.coli JM109	endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ (lac-proAB)	Promega®
E.coli JM109estA	E.coli JM109 with pUCP26estA	This study
Plasmids
pUCP26	Tc R, containing Ori from Pseudomonas, Ori from E. coli	[14]
pUCP26estA	pUCP26 containing the full-length estA from P. aeruginosa PAO1, including its native ribosomal-binding site	This study