Table 1. Summary of changes in protein levels, protein phosphorylation and gene expression in Candida albicans in response to CaPPZ1 gene deletion.
Protein1,2 | Spot ID | RuBPS staining | Pro-Q Diamond staining | Gene expression | |||
---|---|---|---|---|---|---|---|
Ratio | p value | Ratio | p value | Ratio | p value | ||
Group 1 | |||||||
Ade12 | 46 | 2.60 | 0.04 | n.a. | - | 1.09 | 0.82 |
Bmh1 | 47 | -1.97 | 0.01 | n.a. | - | -1.07 | 0.44 |
Efb1 | 43 | -2.37 | 0.002 | n.a. | - | 1.38 | 0.33 |
Idh1 | 45 | 2.76 | 0.02 | n.a. | - | -1.02 | 0.95 |
Ure2 | 48 | 1.95 | 0.0004 | n.a. | - | -1.28 | 0.36 |
Group 2 | |||||||
Cef3* | 44 | -7.08 | 0.004 | n.a. | - | 1.43 | 0.01 |
27 | -3.63 | 0.02 | -2.38 | 0.008 | |||
Cpr3 | 02 | -2.20 | 0.01 | -2.59 | 0.018 | 1.05 | 0.36 |
Hsp70/Ssa1 | 32 | -1.52 | 0.04 | -3.32 | 0.024 | 1.17 | 0.59 |
Rpl20B | 10 | -2.21 | 0.02 | -4.81 | 0.019 | 1.50 | 0.10 |
Tkl1 | 29 | 1.84 | 0.01 | 1.78 | 0.045 | -1.19 | 0.44 |
Tsa1 | 16 | -2.23 | 0.002 | -2.18 | 0.028 | 1.14 | 0.23 |
Yst1 | 22 | -2.35 | 0.04 | -2.79 | 0.041 | 1.79 | 0.06 |
Group 3 | |||||||
Aip2 | 34 | -1.25 | 0.51 | -2.05 | 0.026 | 1.67 | 0.18 |
Cyp5 | 07 | -2.62 | 0.09 | -2.02 | 0.0003 | 1.61 | 0.15 |
Dug1-like | 36 | -1.25 | 0.51 | 1.37 | 0.008 | 1.13 | 0.29 |
Eft2 | 28 | -1.11 | 0.69 | 2.79 | 0.008 | 1.10 | 0.63 |
Guk1 | 03 | -1.84 | 0.13 | -4.57 | 0.003 | 1.87 | 0.05 |
Ham1 | 01 | 1.34 | 0.46 | -2.79 | 0.034 | 1.17 | 0.49 |
Rad23 | 39 | -1.24 | 0.37 | 1.95 | 0.006 | -1.26 | 0.17 |
Rpl9B | 13 | -1.69 | 0.19 | -3.23 | 0.049 | 1.32 | 0.10 |
Rpp0* | 20 | 1.74 | 0.15 | 2.56 | 0.046 | 1.20 | 0.67 |
21 | -1.08 | 0.82 | 2.63 | 0.005 | |||
Rps7A | 04 | -4.98 | 0.12 | -2.58 | 0.047 | 1.22 | 0.03 |
Tif1 | 23 | 1.09 | 0.77 | -2.05 | 0.014 | 1.44 | 0.16 |
Group 4 | |||||||
Lia1-like* | 17 | -1.06 | 0.75 | -3.09 | 0.019 | 1.54 | 0.10 |
18 | -1.59 | 0.02 | -2.32 | 0.008 | |||
Uba1* | 30 | 1.59 | 0.03 | 1.90 | 0.006 | 1.03 | 0.98 |
31 | 1.21 | 0.56 | -2.31 | 0.037 |
* The protein was identified in two separate spots of 2D gels. n.a. not applicable, as no phosphoprotein specific staining can be detected. Significant changes (p < 0.05) are shown in bold face.
Based on