Representative immunoblot data (A) and pooled data (B). Phospholylated AMPKα, ACC and PDH were at Thr172, Ser79 and Ser293, respectively. Group-IR demonstrated higher expression levels of phosphorylated PDH relative to total PDH compared with Group-C. Values are presented as mean ± SE; n=5–6 per group. *P<0.05 vs. Group-C. AMPKα, 5′ adenosine monophosphate-activated protein kinase; ACC, acetyl-CoA carboxylase; PDH, pyruvate dehydrogenase; Group-IR, Ischemia-reperfusion group; Group-C, control group; AU, arbitrary unit.