Antibodies |
|
Rabbit Monoclonal anti-RPS3 |
Cell Signaling Technology |
Cat# 9538 |
Rabbit Polyclonal anti-RPS29 |
Sigma-Aldrich |
Cat# AV40329; RRID:AB_1856467 |
Rabbit Polyclonal anti-RPS18 |
Abcam |
Cat# ab91293; RRID:AB_2050267 |
Rabbit Polyclonal anti-EXOSC10 |
Abcam |
Cat# ab50558; RRID:AB_869937 |
Goat Polyclonal anti-PAPD5 |
Sigma-Aldrich |
Cat# SAB2501846 (lot number 10875P1) |
Mouse Monoclonal anti-RNA PolII F-12 |
Santa Cruz Biotechnology |
Cat# sc-55492 |
Rabbit anti-RNA PolI (RPA-116/RPA2) |
gifted by Prof. Dr. Ingrid Grummt |
N/A |
Mouse Monoclonal anti-U2AF65 |
Sigma-Aldrich |
Cat# U4758 |
Mouse Monoclonal anti-β-Actin |
Sigma-Aldrich |
Cat# A5441 |
Rabbit Polyclonal anti-NOCTURNIN |
Prepared by C.B. Green |
N/A |
Mouse Monoclonal Anti-α-Tubulin |
Sigma-Aldrich |
Cat# T9026 |
B-catenin |
BD Biosciences |
Cat#610154 |
Rabbit anti-phospho-S6 Ribosomal Protein (Ser235/236) |
Cell Signaling Technology |
Cat#2211 |
Rabbit anti-S6 ribosomal Protein (5G10) |
Cell Signaling Technology |
Cat#2217 |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Lipofectamine RNAiMAX |
Thermo Fisher Scientific |
Cat# 13778075 |
Cycloheximide |
Sigma-Aldrich |
Cat# C7698 |
TRIZOL |
Thermo Fisher Scientific |
Cat#15596018 |
|
Critical Commercial Assays |
|
μMACS Streptavidin kit |
Miltenyi Biotec |
Cat# 130-074-101 |
SuperScript II reverse transcriptase |
Thermo Fisher Scientific |
Cat# 18064071 |
SYBR Green Master Mix |
Roche |
Cat# 04707516001 |
|
Deposited Data |
|
Ribosome profiling and RNA-seq data (ad lib-fed mice) |
GEO (http://www.ncbi.nlm.nih.gov/geo/) |
GSE67305 |
Ribosome profiling and RNA-seq data (night-fed mice) |
GEO (http://www.ncbi.nlm.nih.gov/geo) |
GSE73554 |
|
Experimental Models: Cell Lines |
|
NIH 3T3 cell line |
ATCC |
Cat# CRL-6442 RRID:CVCL_0594 |
|
Experimental Models: Organisms/Strains |
|
Mouse: Wild-type C57BL/6JRJ |
Janvier Labs |
RRID:IMSR_JAX:000664 |
Mouse: WT and Nocturnin KO mice (C57BL/6 background) |
Laboratory of C.B. Green |
N/A |
|
Recombinant DNA |
|
Sequence-Based Reagents |
|
ON-TARGET SMART-Pool siRNAs targeting Rps29 sequence |
Dharmacon (GE Healthcare) |
Cat# L-045910 |
ON-TARGET SMART-Pool siRNAs targeting Rps18 sequence |
Dharmacon (GE Healthcare) |
Cat# L-042224 |
ON-TARGET SMART-Pool siRNAs targeting Rps3 sequence |
Dharmacon (GE Healthcare) |
Cat# L-047921 |
ON-TARGET SMART-Pool siRNAs targeting Papd5 sequence |
Dharmacon (GE Healthcare) |
Cat# L-061333 |
ON-TARGET SMART-Pool siRNAs targeting Exosc10 sequence |
Dharmacon (GE Healthcare) |
Cat# L-049286 |
ON-TARGET SMART-Pool siRNAs targeting PARN sequence |
Dharmacon (GE Healthcare) |
Cat# L-040664 |
ON-TARGET SMART-Pool siRNAs targeting Xrn2 sequence |
Dharmacon (GE Healthcare) |
Cat# L-046490 |
See Table S1 for primer list |
This paper |
N/A |
|
Software and Algorithms |
|
ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
MATLAB |
MathWorks |
https://www.mathworks.com |
|
Other |