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. Author manuscript; available in PMC: 2018 May 4.
Published in final edited form as: Cell. 2017 May 4;169(4):651–663.e14. doi: 10.1016/j.cell.2017.04.015
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit Monoclonal anti-RPS3 Cell Signaling Technology Cat# 9538
Rabbit Polyclonal anti-RPS29 Sigma-Aldrich Cat# AV40329; RRID:AB_1856467
Rabbit Polyclonal anti-RPS18 Abcam Cat# ab91293; RRID:AB_2050267
Rabbit Polyclonal anti-EXOSC10 Abcam Cat# ab50558; RRID:AB_869937
Goat Polyclonal anti-PAPD5 Sigma-Aldrich Cat# SAB2501846 (lot number 10875P1)
Mouse Monoclonal anti-RNA PolII F-12 Santa Cruz Biotechnology Cat# sc-55492
Rabbit anti-RNA PolI (RPA-116/RPA2) gifted by Prof. Dr. Ingrid Grummt N/A
Mouse Monoclonal anti-U2AF65 Sigma-Aldrich Cat# U4758
Mouse Monoclonal anti-β-Actin Sigma-Aldrich Cat# A5441
Rabbit Polyclonal anti-NOCTURNIN Prepared by C.B. Green N/A
Mouse Monoclonal Anti-α-Tubulin Sigma-Aldrich Cat# T9026
B-catenin BD Biosciences Cat#610154
Rabbit anti-phospho-S6 Ribosomal Protein (Ser235/236) Cell Signaling Technology Cat#2211
Rabbit anti-S6 ribosomal Protein (5G10) Cell Signaling Technology Cat#2217

Chemicals, Peptides, and Recombinant Proteins

Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778075
Cycloheximide Sigma-Aldrich Cat# C7698
TRIZOL Thermo Fisher Scientific Cat#15596018

Critical Commercial Assays

μMACS Streptavidin kit Miltenyi Biotec Cat# 130-074-101
SuperScript II reverse transcriptase Thermo Fisher Scientific Cat# 18064071
SYBR Green Master Mix Roche Cat# 04707516001

Deposited Data

Ribosome profiling and RNA-seq data (ad lib-fed mice) GEO (http://www.ncbi.nlm.nih.gov/geo/) GSE67305
Ribosome profiling and RNA-seq data (night-fed mice) GEO (http://www.ncbi.nlm.nih.gov/geo) GSE73554

Experimental Models: Cell Lines

NIH 3T3 cell line ATCC Cat# CRL-6442 RRID:CVCL_0594

Experimental Models: Organisms/Strains

Mouse: Wild-type C57BL/6JRJ Janvier Labs RRID:IMSR_JAX:000664
Mouse: WT and Nocturnin KO mice (C57BL/6 background) Laboratory of C.B. Green N/A

Recombinant DNA

Sequence-Based Reagents

ON-TARGET SMART-Pool siRNAs targeting Rps29 sequence Dharmacon (GE Healthcare) Cat# L-045910
ON-TARGET SMART-Pool siRNAs targeting Rps18 sequence Dharmacon (GE Healthcare) Cat# L-042224
ON-TARGET SMART-Pool siRNAs targeting Rps3 sequence Dharmacon (GE Healthcare) Cat# L-047921
ON-TARGET SMART-Pool siRNAs targeting Papd5 sequence Dharmacon (GE Healthcare) Cat# L-061333
ON-TARGET SMART-Pool siRNAs targeting Exosc10 sequence Dharmacon (GE Healthcare) Cat# L-049286
ON-TARGET SMART-Pool siRNAs targeting PARN sequence Dharmacon (GE Healthcare) Cat# L-040664
ON-TARGET SMART-Pool siRNAs targeting Xrn2 sequence Dharmacon (GE Healthcare) Cat# L-046490
See Table S1 for primer list This paper N/A

Software and Algorithms

ImageJ NIH https://imagej.nih.gov/ij/
MATLAB MathWorks https://www.mathworks.com

Other