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. 2017 Aug 24;10(5):793–799. doi: 10.1016/j.tranon.2017.07.009

Figure 4.

Figure 4

Treatment effect on mTOR signaling and expression of p21 in the liver of Tsc1+/− mice.

(A) Western analysis. Proteins were prepared from liver tissues dissected from Tsc1+/− mice treated for 2 months with vehicle, atorvastatin, rapamycin, or atorvastatin/rapamycin combination. β-Actin was used as a loading control. Representative Western blots were presented to show phosphorylation of Akt at S473 and S6 at S235/236 and expression of p21.

(B) q-PCR analysis. Total RNA was isolated from liver tissues dissected from Tsc1+/− mice treated for 2 months with vehicle, atorvastatin, rapamycin, or atorvastatin/rapamycin combination (n = 5 each group). RNA was used to synthesize cDNA, and real time q-PCR was performed to estimate relative quantity of Cdkn1a transcripts.