Extended Data Figure 6. The roles of GATOR2 components in SZT2 regulation of mTORC1 signaling.

a – c, Cells were deprived of amino acids for 1 h and stimulated with amino acids for 10 min when indicated. Total cell lysates were analyzed by immunoblotting. d, Control or SZT2-deficient cells were transfected with LentiCrisprV2 plasmids targeting GFP or SEH1L, selected with puromycin, and analyzed as in (a). Data (a – d) are representatives of three independent experiments.