Extended Data Figure 8. Lysosome-targeted WDR59 or Sestrin2 suppresses mTORC1 signaling in GATOR1-deficient cells.

a, b and d, Cells were transfected with the indicated constructs, and selected with puromycin. Cells were deprived of amino acids for 1 h and stimulated with amino acids for 20 min when indicated. Total cell lysates were analyzed by immunoblotting. c and e, Cells were deprived of amino acids for 1 h and stimulated with amino acids for 20 min when indicated. Total cell lysates were analyzed by immunoblotting. Data (a – e) are representatives of three independent experiments.